A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy

I M Sulaiman; N Z Ehtesham; S E Hasnain

doi:10.1016/0378-1119(95)00087-m

A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy

Gene. 1995 Apr 24;156(2):223-7. doi: 10.1016/0378-1119(95)00087-m.

Authors

I M Sulaiman¹, N Z Ehtesham, S E Hasnain

Affiliation

¹ Eukaryotic Gene Expression Lab, National Institute of Immunology, New Delhi, India.

PMID: 7758960
DOI: 10.1016/0378-1119(95)00087-m

Abstract

Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Conservation of Natural Resources
DNA Probes*
DNA, Plant / genetics*
Nucleic Acid Hybridization
Phylogeny
Plants / classification*
Plants / genetics*
Polymorphism, Restriction Fragment Length*
Repetitive Sequences, Nucleic Acid / genetics

Substances

DNA Probes
DNA, Plant