In order to establish a clinical or laboratory difference 52 Clinical charts of patients that were attended at the Gastroenterologic Unit at the Children's Hospital in Lima-Perú since January 1988 to December 1992, with the diagnosis of Neonatal Cholestasis were reviewed. 14 of them were discharged; at the end we have reviewed 23 cases of biliary atresia, 8 of neonatal hepatitis and 7 with congenital choledochal cyst. All patients went through sonography, scintigraphy and liver biopsy. No statistical differences were found in symptomatology, as well as jaundice, feces color or coluria. We concluded that the most accurate way to predict a diagnosis is through the sonography, scintigraphy and liver biopsy.