Pulsed-field gel analysis of 5S and satellite DNA in barley

Genome. 1995 Feb;38(1):153-7. doi: 10.1139/g95-019.

Abstract

Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers / genetics
  • DNA, Plant / genetics*
  • DNA, Ribosomal / genetics
  • DNA, Satellite / genetics*
  • Genetic Markers
  • Hordeum / genetics*
  • Minisatellite Repeats
  • Molecular Sequence Data
  • Multigene Family
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 5S / genetics
  • Repetitive Sequences, Nucleic Acid

Substances

  • DNA Primers
  • DNA, Plant
  • DNA, Ribosomal
  • DNA, Satellite
  • Genetic Markers
  • RNA, Ribosomal, 5S