Abstract
Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers / genetics
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DNA, Plant / genetics*
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DNA, Ribosomal / genetics
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DNA, Satellite / genetics*
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Genetic Markers
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Hordeum / genetics*
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Minisatellite Repeats
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Molecular Sequence Data
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Multigene Family
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 5S / genetics
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Repetitive Sequences, Nucleic Acid
Substances
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DNA Primers
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DNA, Plant
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DNA, Ribosomal
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DNA, Satellite
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Genetic Markers
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RNA, Ribosomal, 5S