[The genetic polymorphism of cereals demonstrated by PCR with random primers]

Tsitol Genet. 1994 Nov-Dec;28(6):54-61.
[Article in Russian]

Abstract

Polymerase chain reaction of DNA amplification with the use of random primers is a new approach in investigations of genome specificity. Nucleotide sequences that showed polymorphism in RFLP analysis were used as primers. Optimal temperature conditions and Mg2+ concentrations were determined for studying inter- and intraspecific DNA polymorphism in the most important cereals.

Publication types

  • English Abstract

MeSH terms

  • Base Sequence
  • DNA Primers / genetics*
  • DNA, Plant / genetics
  • Edible Grain / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic / genetics*
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Plant