Effect of delayed specimen processing on cytomegalovirus antigenemia test results

J Clin Microbiol. 1995 Jan;33(1):257-9. doi: 10.1128/jcm.33.1.257-259.1995.

Abstract

Blood samples held at either 4 degrees C or room temperature for 1 day had similar mean decreases in number of cytomegalovirus antigenemia-positive cells (52 to 55%) and similar false-negative test results (13 to 14%). After 2 days, samples held at 4 degrees C showed no further decline, whereas samples held at room temperature had a mean 81% decrease in positive cells, a 32% false-negative rate, and a more marked deterioration in cell morphology.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral / blood*
  • Bone Marrow Transplantation
  • Cold Temperature
  • Cytomegalovirus Infections / blood
  • Cytomegalovirus Infections / complications
  • Cytomegalovirus Infections / diagnosis*
  • False Negative Reactions
  • Fluorescent Antibody Technique
  • HIV Infections / complications
  • Hot Temperature
  • Humans
  • Leukocytes / virology
  • Phosphoproteins / blood
  • Specimen Handling*
  • Time Factors
  • Viral Matrix Proteins / blood

Substances

  • Antigens, Viral
  • Phosphoproteins
  • Viral Matrix Proteins
  • cytomegalovirus matrix protein 65kDa