Abstract
Profilin is a low molecular weight protein involved in the organization of the mammalian and protozoan cytoskeleton as well as in signal transduction. In this study, profilin is identified as an actin-binding protein in higher plants which is present in monocot and dicot angiosperms. Birch pollen profilin and actin can be copurified as a complex, and purified recombinant birch profilin can be used as an affinity matrix to obtain birch pollen actin. The binding of 125I-labeled recombinant birch pollen profilin to plant and animal actins can be blocked by profilin-specific antibodies that react with different epitopes of birch profilin. One of the blocking antibodies was raised against the 25 COOH-terminal amino acids indicating the importance of this region in the profilactin complex formation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Amino Acid Sequence
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Animals
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Antibody Specificity
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Cloning, Molecular
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Contractile Proteins*
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Electrophoresis, Polyacrylamide Gel
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Epitopes / analysis
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Humans
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Microfilament Proteins / biosynthesis
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Microfilament Proteins / isolation & purification
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Microfilament Proteins / metabolism*
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Molecular Sequence Data
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Molecular Weight
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Peptides / chemical synthesis
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Plants / metabolism*
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Poaceae / metabolism
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Pollen / chemistry
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Profilins
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Trees / metabolism
Substances
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Actins
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Contractile Proteins
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Epitopes
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Microfilament Proteins
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Peptides
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Profilins
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Recombinant Proteins