HeLa D98/aH-2 studied by chromosome painting and conventional cytogenetical techniques

Chromosoma. 1993 Jul;102(7):473-7. doi: 10.1007/BF00357102.

Abstract

Metaphase chromosomes of HeLa D98/AH-2 cells were studied by GTG-banding, NOR-silver staining and in situ hybridization using libraries specific for each human chromosome as probes (chromosome painting). The structure and composition of all marker chromosomes could be determined, allowing a critical assessment of earlier studies. The revised HeLa D98/AH-2 karyotype should provide a reference standard in certain cancer cytogenetic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Banding
  • Chromosomes, Human*
  • Cytogenetics
  • Genetic Markers
  • Genetic Techniques
  • HeLa Cells / ultrastructure*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Staining and Labeling

Substances

  • Genetic Markers