Abstract
Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs, IB8 (anti-HA1) and IIF4 (anti-HA2) respectively, revealed that HA2 epitope was reachable for antibody only in minor subpopulation of the HA representing approximately 7% of all molecules (spikes). The basis of the HA heterogeneity is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Antibody Specificity
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Binding, Competitive
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Cell Line
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Cells, Cultured
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Chick Embryo
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Dogs
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Epitopes / analysis*
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral / analysis*
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Hemagglutinins, Viral / immunology*
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Influenza A virus / immunology*
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Influenza A virus / ultrastructure
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Kidney
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Kinetics
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Microscopy, Electron
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Microscopy, Immunoelectron
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Radioimmunoassay
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Viral Envelope Proteins / analysis
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Monoclonal
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Epitopes
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral
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Viral Envelope Proteins