Monoclonal antibodies demonstrate accessible HA2 epitopes in minor subpopulation of native influenza virus haemagglutinin molecules

Arch Virol. 1993;130(1-2):45-56. doi: 10.1007/BF01318995.

Abstract

Haemagglutinin (HA) was detected on the surface of influenza virus infected cell with monoclonal antibodies (MAbs) against both HA glycopolypeptides, HA1 and HA2, however, the reactivity of HA2-specific MAbs was remarkably lower as compared with HA1-specific MAbs. Quantitative analysis with two MAbs, IB8 (anti-HA1) and IIF4 (anti-HA2) respectively, revealed that HA2 epitope was reachable for antibody only in minor subpopulation of the HA representing approximately 7% of all molecules (spikes). The basis of the HA heterogeneity is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibody Specificity
  • Binding, Competitive
  • Cell Line
  • Cells, Cultured
  • Chick Embryo
  • Dogs
  • Epitopes / analysis*
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / analysis*
  • Hemagglutinins, Viral / immunology*
  • Influenza A virus / immunology*
  • Influenza A virus / ultrastructure
  • Kidney
  • Kinetics
  • Microscopy, Electron
  • Microscopy, Immunoelectron
  • Radioimmunoassay
  • Viral Envelope Proteins / analysis
  • Viral Envelope Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • Viral Envelope Proteins