Abstract
A hepatitis B core antigen (HBcAg) gene bearing the 39-amino-acid-long domain A of hepatitis B surface antigen (HBsAg) within the HBcAg immunodominant loop has been constructed and expressed in Escherichia coli. Chimeric capsids demonstrated HBs but not HBc antigenicity and elicited in mice B-cell and T-cell responses against native HBcAg and HBsAg.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / immunology
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Base Sequence
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Capsid / biosynthesis
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Capsid / genetics
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Capsid / immunology*
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Epitopes / immunology
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Escherichia coli / genetics
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Hepatitis B Antibodies / immunology
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Hepatitis B Core Antigens / biosynthesis
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Hepatitis B Core Antigens / genetics
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Hepatitis B Core Antigens / immunology*
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Hepatitis B Surface Antigens / biosynthesis
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Hepatitis B Surface Antigens / genetics
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Hepatitis B Surface Antigens / immunology*
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Hepatitis B virus / genetics
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Hepatitis B virus / immunology*
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Immunodominant Epitopes / genetics*
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Immunodominant Epitopes / immunology
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Mice
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Molecular Sequence Data
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Rabbits
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / immunology
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T-Lymphocytes / immunology
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T-Lymphocytes, Helper-Inducer / immunology
Substances
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Epitopes
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Hepatitis B Antibodies
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Hepatitis B Core Antigens
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Hepatitis B Surface Antigens
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Immunodominant Epitopes
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Recombinant Proteins