Multiple genes code for high-molecular-mass rhoptry proteins of Plasmodium yoelii

Mol Biochem Parasitol. 1995 Mar;70(1-2):149-55. doi: 10.1016/0166-6851(95)00025-v.

Abstract

We have examined the number of genes coding for a group of high-molecular-mass rhoptry protein(s) in the malaria parasite Plasmodium yoelii, and studied variation in the gene family within the parasite's genome. A region of the genes was amplified using oligonucleotides based on conserved DNA sequences and the products cloned. The sequences could be divided into 7 groups by restriction-fragment-length polymorphism. Further variation was detected by sequence analysis; 11 different sequences were detected in the 16 clones analyzed. The genes in the family were distributed on 6 chromosomes probably at 9 or more loci.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Genes, Protozoan*
  • Genetic Variation
  • Mice
  • Molecular Sequence Data
  • Plasmodium yoelii / genetics*
  • Plasmodium yoelii / immunology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / immunology
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid

Substances

  • Protozoan Proteins

Associated data

  • GENBANK/U19979
  • GENBANK/U19980
  • GENBANK/U19981
  • GENBANK/U19982
  • GENBANK/U19983
  • GENBANK/U19984
  • GENBANK/U19985
  • GENBANK/U19986
  • GENBANK/U19987
  • GENBANK/U19988
  • GENBANK/U19989
  • GENBANK/U19990
  • GENBANK/U19991
  • GENBANK/U19992