Nitric oxide production and mycobacterial growth inhibition by murine alveolar macrophages: the sequence of rIFN-gamma stimulation and Mycobacterium bovis BCG infection determines macrophage activation

R Hanano; S H Kaufmann

doi:10.1016/0165-2478(94)00193-u

Nitric oxide production and mycobacterial growth inhibition by murine alveolar macrophages: the sequence of rIFN-gamma stimulation and Mycobacterium bovis BCG infection determines macrophage activation

Immunol Lett. 1995 Feb;45(1-2):23-7. doi: 10.1016/0165-2478(94)00193-u.

Authors

R Hanano¹, S H Kaufmann

Affiliation

¹ Department of Immunology, University of Ulm, Germany.

PMID: 7622183
DOI: 10.1016/0165-2478(94)00193-u

Abstract

Prestimulation with recombinant-interferon-gamma (rIFN-gamma) followed by Mycobacterium bovis BCG infection induced high nitric oxide production and potent mycobacterial growth inhibition in murine alveolar macrophages. Reversal of the sequence of treatments caused opposite effects, suggesting that a sequential 2-step process comprising first rIFN-gamma stimulation and second mycobacterial infection is operative in the activation of alveolar macrophages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers
Interferon-gamma / pharmacology
Macrophage Activation / drug effects*
Macrophages, Alveolar / metabolism
Macrophages, Alveolar / physiology*
Mice
Mice, Inbred C57BL
Mycobacterium bovis* / growth & development
NADPH Dehydrogenase / analysis
Nitric Oxide / biosynthesis*
Recombinant Proteins
Specific Pathogen-Free Organisms
Tuberculosis / immunology
Tuberculosis / microbiology

Substances

Biomarkers
Recombinant Proteins
Nitric Oxide
Interferon-gamma
NADPH Dehydrogenase