Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation

S Izumi; A Manabe; A Tomoyasu; F Kihara-Negishi; H Ariga

doi:10.1016/0167-4889(95)00071-y

Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation

Biochim Biophys Acta. 1995 Jun 20;1267(2-3):135-8. doi: 10.1016/0167-4889(95)00071-y.

Authors

S Izumi¹, A Manabe, A Tomoyasu, F Kihara-Negishi, H Ariga

Affiliation

¹ Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.

PMID: 7612666
DOI: 10.1016/0167-4889(95)00071-y

Abstract

A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cell Differentiation
Cell Line
Cloning, Molecular
DNA, Complementary / metabolism*
Gene Expression
Mice
Molecular Sequence Data
Pyruvate Kinase / genetics*

Substances

DNA, Complementary
Pyruvate Kinase

Associated data

GENBANK/D38379