New restriction endonucleases from thermophilic soil bacteria

V E Repin; L R Lebedev; L Puchkova; G D Serov; T Tereschenko; V E Chizikov; I Andreeva

doi:10.1016/0378-1119(94)00781-m

New restriction endonucleases from thermophilic soil bacteria

Gene. 1995 May 19;157(1-2):321-2. doi: 10.1016/0378-1119(94)00781-m.

Authors

V E Repin¹, L R Lebedev, L Puchkova, G D Serov, T Tereschenko, V E Chizikov, I Andreeva

Affiliation

¹ State Research Center-ESP Vektor, Koltzovo, Novosibirsk region, Russia.

PMID: 7607519
DOI: 10.1016/0378-1119(94)00781-m

Abstract

Using the most effective rapid method for the detection of restriction endonucleases (ENases) in microorganisms, 32 thermophilic producers have been isolated. All strains belong to the genus Bacillus. Thermostable isoschizomers of ENases, such as AvaI, BbvI, BbvII, BclI, BsaBI, BsiYI, BsrI, BstEII, BstNI, Cfr10I, ClaI, FspI, HaeIII, HpaII, Ksp632I and SfeI, were isolated.

Publication types

Comparative Study

MeSH terms

Bacillus / enzymology*
Base Sequence
DNA Restriction Enzymes / isolation & purification
DNA Restriction Enzymes / metabolism*
Enzyme Stability
Hot Temperature
Molecular Sequence Data
Soil Microbiology*
Species Specificity

Substances

DNA Restriction Enzymes