Abstract
Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.
MeSH terms
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Base Sequence
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Colon / microbiology
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Escherichia coli / genetics*
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Escherichia coli / growth & development*
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Escherichia coli / metabolism
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Escherichia coli Proteins
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Gene Expression Regulation, Bacterial*
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Humans
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Methyltransferases / biosynthesis*
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Methyltransferases / genetics
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Promoter Regions, Genetic*
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Recombinant Fusion Proteins / biosynthesis
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Site-Specific DNA-Methyltransferase (Adenine-Specific)*
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Substrate Specificity
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beta-Galactosidase / biosynthesis
Substances
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Escherichia coli Proteins
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Recombinant Fusion Proteins
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Methyltransferases
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Dam methyltransferase
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Site-Specific DNA-Methyltransferase (Adenine-Specific)
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dam protein, E coli
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beta-Galactosidase