Growth-rate-dependent transcription initiation from the dam P2 promoter

Gene. 1995 May 19;157(1-2):213-5. doi: 10.1016/0378-1119(94)00619-4.

Abstract

Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.

MeSH terms

  • Base Sequence
  • Colon / microbiology
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development*
  • Escherichia coli / metabolism
  • Escherichia coli Proteins
  • Gene Expression Regulation, Bacterial*
  • Humans
  • Methyltransferases / biosynthesis*
  • Methyltransferases / genetics
  • Promoter Regions, Genetic*
  • Recombinant Fusion Proteins / biosynthesis
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)*
  • Substrate Specificity
  • beta-Galactosidase / biosynthesis

Substances

  • Escherichia coli Proteins
  • Recombinant Fusion Proteins
  • Methyltransferases
  • Dam methyltransferase
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • dam protein, E coli
  • beta-Galactosidase