Abstract
cDNAs for four different forms of syntaxin 3 were cloned from a mouse brain cDNA library, and the proteins encoded by these clones were named syntaxin 3A (previous syntaxin 3), 3B, 3C and 3D. Syntaxin 3B contained a different sequence in the carboxyl terminal region from that of syntaxin 3A. The amino terminal region of syntaxin 3C contained an 18 amino acid sequence instead of a 34 amino acid sequence present in syntaxins 3A and 3B. Syntaxin 3D consisted of only 86 amino acids and lacked any putative transmembrane segments. These forms of syntaxin 3 are probably generated by alternative splicing of the primary transcript of syntaxin 3 gene. Cytoplasmic portions of syntaxins 3A and 3B but not of syntaxin 3C or 3D bound to Munc-18/n-sec1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain Chemistry / genetics*
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Cloning, Molecular
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DNA, Complementary / genetics
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mice
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Molecular Sequence Data
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Munc18 Proteins
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Polymerase Chain Reaction
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Protein Binding
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Qa-SNARE Proteins
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Vesicular Transport Proteins*
Substances
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DNA, Complementary
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Membrane Proteins
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Munc18 Proteins
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Nerve Tissue Proteins
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Qa-SNARE Proteins
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Recombinant Proteins
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Vesicular Transport Proteins
Associated data
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GENBANK/D29743
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GENBANK/D29797
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GENBANK/D29798
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GENBANK/D29799
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GENBANK/D29800
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GENBANK/D38375