Revision of the amino-acid sequence of 3-isopropylmalate dehydrogenase from Salmonella typhimurium by means of X-ray crystallography

Gene. 1995 Oct 16;164(1):85-7. doi: 10.1016/0378-1119(95)00494-q.

Abstract

The amino acid (aa) sequence of the leuB gene product of Salmonella typhimurium, 3-isopropylmalate dehydrogenase (IPMDH), has been revised using electron density maps from X-ray structure determination. The nucleotide (nt) sequence of both strands of leuB has been redetermined to confirm the crystallographic findings. It does not agree with the previously reported S. typhimurium leuB nucleotide sequence [Andreadis and Rosenthal, Biochim. Biophys. Acta 1129 (1992) 228-230].

Publication types

  • Comparative Study

MeSH terms

  • 3-Isopropylmalate Dehydrogenase
  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Artifacts
  • Base Sequence
  • Crystallography, X-Ray
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Salmonella typhimurium / enzymology
  • Salmonella typhimurium / genetics*
  • Sequence Analysis, DNA

Substances

  • Alcohol Oxidoreductases
  • 3-Isopropylmalate Dehydrogenase

Associated data

  • GENBANK/S76460
  • GENBANK/U20795
  • GENBANK/X53376