Aspirin inhibits collagen-induced platelet serotonin release, as measured by microbore high-performance liquid chromatography with electrochemical detection

J Chromatogr B Biomed Appl. 1995 Jul 21;669(2):404-7. doi: 10.1016/0378-4347(95)00119-4.

Abstract

A sensitive microbore high-performance liquid chromatographic method with electrochemical detection has been developed for the measurement of small quantities of serotonin (5-hydroxytryptamine; 5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in rabbit platelets. The limit of detection of 5-HT is 0.1 ng/ml. To evaluate the 5-HT release from platelet suspension, the aggregating agent collagen was added at concentrations of 10, 30 and 100 micrograms/ml and the 5-HT concentrations rose from the base level (11.80 +/- 0.42 ng/ml) to 123.97 +/- 11.02, 361.96 +/- 17.90 and 470.45 +/- 35.46 ng/ml, respectively. Further results demonstrated that aspirin inhibits collagen-induced 5-HT release from platelet suspension significantly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aspirin / pharmacology*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism
  • Chromatography, High Pressure Liquid / methods*
  • Collagen / antagonists & inhibitors*
  • Collagen / pharmacology
  • Electrochemistry
  • Platelet Aggregation Inhibitors / pharmacology*
  • Rabbits
  • Reproducibility of Results
  • Serotonin / metabolism*

Substances

  • Platelet Aggregation Inhibitors
  • Serotonin
  • Collagen
  • Aspirin