PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes

PCR Methods Appl. 1994 Oct;4(2):85-8. doi: 10.1101/gr.4.2.85.

Abstract

The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Ferritins / genetics*
  • Gene Deletion
  • Humans
  • Introns
  • Molecular Sequence Data
  • Multigene Family*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • Pseudogenes*

Substances

  • DNA, Complementary
  • Ferritins

Associated data

  • GENBANK/X80334
  • GENBANK/X80335
  • GENBANK/X80337