An enzyme-linked immunosorbent assay (ELISA) was used to detect T. spiralis infection in dogs, using larval T. spiralis excretory-secretory (ES) antigen. Forty-three (4.3%) dog sera out of 1000 revealed the presence of IgG T. spiralis. The positive sera were distributed in three groups; 21 (2.1%) weakly positive, 14 (1.4%) moderately positive, and eight (0.8%) strongly positive.