Abstract
One of three gene copies encoding ammonia monooxygenase subunit A (AmoA) and flanking sequences was isolated from genomic DNA of the chemolithotrophic soil bacterium Nitrosospira sp. NpAV using oligodeoxyribonucleotide primers and the polymerase chain reaction (PCR). The gene (amoA) encodes a 274-amino-acid polypeptide which has similarity to the ammonia monooxygenase acetylene-binding protein from Nitrosomonas europaea.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA Primers
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DNA, Bacterial / chemistry
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DNA, Bacterial / metabolism
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Genes, Bacterial*
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Macromolecular Substances
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Molecular Sequence Data
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Nitrosomonas / genetics
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Oxidoreductases / biosynthesis
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Oxidoreductases / genetics*
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Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Soil Microbiology
Substances
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DNA Primers
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DNA, Bacterial
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Macromolecular Substances
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Oxidoreductases
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ammonia monooxygenase
Associated data
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GENBANK/U20644
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GENBANK/U38250
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GENBANK/U38251