Sequence of an ammonia monooxygenase subunit A-encoding gene from Nitrosospira sp. NpAV

Gene. 1995 Sep 22;163(1):159-60. doi: 10.1016/0378-1119(95)00392-j.

Abstract

One of three gene copies encoding ammonia monooxygenase subunit A (AmoA) and flanking sequences was isolated from genomic DNA of the chemolithotrophic soil bacterium Nitrosospira sp. NpAV using oligodeoxyribonucleotide primers and the polymerase chain reaction (PCR). The gene (amoA) encodes a 274-amino-acid polypeptide which has similarity to the ammonia monooxygenase acetylene-binding protein from Nitrosomonas europaea.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Primers
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / metabolism
  • Genes, Bacterial*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Nitrosomonas / genetics
  • Oxidoreductases / biosynthesis
  • Oxidoreductases / genetics*
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Soil Microbiology

Substances

  • DNA Primers
  • DNA, Bacterial
  • Macromolecular Substances
  • Oxidoreductases
  • ammonia monooxygenase

Associated data

  • GENBANK/U20644
  • GENBANK/U38250
  • GENBANK/U38251