Complete amino acid sequence of the protease inhibitor from buckwheat seeds

M A Belozersky; Y E Dunaevsky; A X Musolyamov; T A Egorov

doi:10.1016/0014-5793(95)00899-k

Complete amino acid sequence of the protease inhibitor from buckwheat seeds

FEBS Lett. 1995 Sep 11;371(3):264-6. doi: 10.1016/0014-5793(95)00899-k.

Authors

M A Belozersky¹, Y E Dunaevsky, A X Musolyamov, T A Egorov

Affiliation

¹ A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.

PMID: 7556606
DOI: 10.1016/0014-5793(95)00899-k

Abstract

The complete amino acid sequence of protease inhibitor BWI-1 from buckwheat (Fagopyrum esculentum Moench) seeds has been established by automatic Edman degradation and mass spectrometry. The molecule of the inhibitor consists of 69 amino acid residues, with a molecular mass calculated as 7743.8 Da. The active site of the inhibitor contains an Arg45-Asp46 bond. Analysis of the amino acid sequence suggests that the buckwheat seed protease inhibitor is a member of the proteinase inhibitor I family.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acids / analysis
Edible Grain / chemistry*
Molecular Sequence Data
Plant Proteins / chemistry*
Seeds / chemistry
Trypsin Inhibitors / chemistry*

Substances

Amino Acids
BWI-1 protein, Fagopyrum esculentum
Plant Proteins
Trypsin Inhibitors