Abstract
Mouse-transformed epidermal cell line (Pam 212) generated the soluble mediators for promoting the growth of a mast cell line (MC9) in the presence of retinoic acid at a concentration of 10(-6)-10(-7) M. The effective molecule of MC9 cell growth promoting factor (MC9-GF) was non-dialyzable and eluted between the molecular weight of 45 K and 68 K on a TSK 2000 G column. Chromatofocusing analysis revealed that this factor had a pI range between 7.0 and 7.5. Anti-c-kit ligand antibody abrogated MC9-GF activity and RT-PCR analysis demonstrated that retinoic acid upregulates c-kit ligand mRNA expression by Pam cells. Several recombinant cytokines including IL1-alpha, IL-1 beta, IL-2, IL-3 or IL-4 did not promote MC9 cell growth at a concentration of 100 U/ml. The presence of anti-IL-1 alpha, -IL-1 beta, -IL-2, -IL-3 or -IL-4 antibodies did not abrogate the MC9-GF activity except for anti-c-kit ligand antibody.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Cytokines / pharmacology
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DNA / genetics
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Growth Substances / biosynthesis
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Hyperplasia
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In Vitro Techniques
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Keratinocytes / drug effects*
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Keratinocytes / metabolism*
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Mast Cells / drug effects
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Mast Cells / pathology
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Polymerase Chain Reaction
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-kit
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor Protein-Tyrosine Kinases / biosynthesis*
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Receptor Protein-Tyrosine Kinases / genetics
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Receptors, Colony-Stimulating Factor / biosynthesis*
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Receptors, Colony-Stimulating Factor / genetics
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Skin / drug effects
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Skin / pathology
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Tretinoin / pharmacology*
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Up-Regulation / drug effects
Substances
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Cytokines
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Growth Substances
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Colony-Stimulating Factor
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Tretinoin
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DNA
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Proto-Oncogene Proteins c-kit
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Receptor Protein-Tyrosine Kinases