The retinohypothalamic tract (RHT) in the Djungarian hamster Phodopus sungorus was studied using anterograde neuronal transport of cholera toxin subunit B (CTB) following unilateral intraocular injection. A major projection terminates bilaterally in the hypothalamic suprachiasmatic nuclei (SCN). In the anterior ventral SCN, a light ipsilateral predominance was evident. In the medial SCN, labelling was concentrated laterally where it was seen over the dorso-ventral extension of the nuclei, pronounced contralaterally to the site of CTB injection, which was even more characteristic in the posterior aspects of the nuclei. Labelled fibers and terminals were observed in the supraoptic nuclei, but not in lateral and paraventricular hypothalamic regions. Additional experiments utilizing double immunofluorescence of CTB and of substance P (SP) in the SCN showed that SP-containing perikarya were particularly observed in a central portion of the nucleus, where CTB-stained terminals were accumulated in the vicinity of immunoreactive cell bodies, fibers and terminals. Our data provide preliminary morphological evidence for the regulation of SCN function by retinal afferents and may explain the circadian and photoperiodic fluctuations in the amount of SP in the SCN.