Peptides for inducing cross-reactive anti-malarial antibodies

Abstract

Immunological cross-reactions between malarial proteins are frequently observed. This paper describes an approach to generate such antibody responses that target many parasite proteins using a limited number of peptides as immunogens. Peptides used contained a known epitope, NKND, that is common to many malarial proteins or combinations of tri-peptides which are commonly present in parasite proteins. One of the two NKND-containing peptides elicited antibodies reacting to six parasite proteins and fusion proteins containing NKND. The antibody specificity was directed to NKND. Two of the four combination peptides were recognized by hyperimmune human sera and mouse immune serum in vitro, and one elicited antibodies recognizing parasites on immunofluorescence assay.