Stabilization of inducible nitric oxide synthase by monoclonal antibodies

R Hattori; K Kosuga; H Eizawa; K Sase; R Inoue; M Sunamoto; Y Ichimori; K Sato; T Mori; K Takahashi

doi:10.1089/hyb.1993.12.763

Stabilization of inducible nitric oxide synthase by monoclonal antibodies

Hybridoma. 1993 Dec;12(6):763-70. doi: 10.1089/hyb.1993.12.763.

Authors

R Hattori¹, K Kosuga, H Eizawa, K Sase, R Inoue, M Sunamoto, Y Ichimori, K Sato, T Mori, K Takahashi, et al.

Affiliation

¹ Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

PMID: 7507086
DOI: 10.1089/hyb.1993.12.763

Abstract

We have produced 14 monoclonal antibodies to inducible nitric oxide synthase purified from rat peritoneal cytotoxic activated macrophages. None of the antibodies showed neutralizing activity, but some of them enhanced the enzyme activity through stabilization of the enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Oxidoreductases / biosynthesis*
Amino Acid Oxidoreductases / immunology
Animals
Antibodies, Monoclonal / immunology*
Blotting, Western
Enzyme Induction
Female
Humans
Hybridomas
Immunohistochemistry
Infant
Mice
Mice, Inbred BALB C
Nitric Oxide Synthase
Rats
Rats, Wistar

Substances

Antibodies, Monoclonal
Nitric Oxide Synthase
Amino Acid Oxidoreductases