Abstract
Components of a signaling pathway that couples the ciliary neurotrophic factor (CNTF) receptor to induction of transcription were identified. CNTF stimulated the tyrosine phosphorylation of p91, a protein implicated in interferon signaling pathways, and of two proteins that are distinct but related to p91. Tyrosine-phosphorylated p91 translocated to the nucleus, where p91 and p91-related proteins bound to a DNA sequence found in promoters of genes responsive to CNTF. This DNA sequence, when inserted upstream of a reporter gene, conferred a transcriptional response to CNTF. A pathway that transduces interferon signals may therefore have a more general function in the propagation of responses to certain neurotrophic factors.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Base Sequence
-
Biological Transport / physiology
-
Cell Nucleus / metabolism*
-
Ciliary Neurotrophic Factor
-
DNA-Binding Proteins / biosynthesis
-
Fibroblast Growth Factor 2 / physiology
-
Gene Expression Regulation / physiology
-
HeLa Cells
-
Humans
-
Interferon-alpha / physiology
-
Interferon-gamma / physiology
-
Molecular Sequence Data
-
Molecular Weight
-
Nerve Tissue Proteins / physiology*
-
Phosphoproteins / biosynthesis
-
Phosphoproteins / chemistry
-
Phosphotyrosine
-
Regulatory Sequences, Nucleic Acid / physiology
-
Signal Transduction / physiology*
-
Transcription, Genetic / physiology*
-
Tumor Cells, Cultured
-
Tyrosine / analogs & derivatives
-
Tyrosine / analysis
Substances
-
Ciliary Neurotrophic Factor
-
DNA-Binding Proteins
-
Interferon-alpha
-
Nerve Tissue Proteins
-
Phosphoproteins
-
Fibroblast Growth Factor 2
-
Phosphotyrosine
-
Tyrosine
-
Interferon-gamma