Genetic and molecular analysis of the rpoD gene from Lactococcus lactis

Biosci Biotechnol Biochem. 1993 Jan;57(1):88-92. doi: 10.1271/bbb.57.88.

Abstract

A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal sigma factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis sigma 43 factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / chemistry
  • Bacillus subtilis / genetics
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Primase
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / genetics*
  • Escherichia coli / chemistry
  • Escherichia coli / genetics
  • Genes, Bacterial
  • Genetic Complementation Test
  • Lactococcus lactis / chemistry
  • Lactococcus lactis / genetics*
  • Molecular Sequence Data
  • Mutation
  • Operon
  • RNA Nucleotidyltransferases / chemistry
  • RNA Nucleotidyltransferases / genetics
  • Restriction Mapping
  • Sigma Factor / chemistry
  • Sigma Factor / genetics*
  • Temperature

Substances

  • Bacterial Proteins
  • Sigma Factor
  • DNA Primase
  • RNA Nucleotidyltransferases
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases

Associated data

  • GENBANK/D10168