A novel factor, TA20, involved in neuronal differentiation: cDNA cloning and expression

Neurosci Res. 1995 Aug;23(1):21-7.

Abstract

Combined treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) and dibutyryl cyclic AMP (diBu-cAMP) induced significantly longer neurites than treatment with each alone in NG108-15 cells. We performed differential screening to identify genes expressed only by treatment with TPA plus diBu-cAMP but not by that with diBu-cAMP for 72 h alone in NG108-15 cells, and isolated a novel gene, TA20. Over-expression of the gene in NG108-15 and neuroblastoma N18TG-2 cells caused intense neurite elongation and suppressed cell growth. TA20 did not cause, however, any morphological changes in glioma C6Bu-1 cells. These results suggest that TA20 is a novel neuronal differentiation factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Differentiation*
  • Cells, Cultured
  • Cloning, Molecular*
  • Cyclic AMP / pharmacology
  • DNA, Complementary / genetics*
  • Gene Expression
  • Genes / physiology*
  • Neurites / physiology*
  • RNA, Messenger / biosynthesis
  • Transfection

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Cyclic AMP