Abstract
The turmeric anti-oxidant protein (TAP) had been isolated from the aqueous extract of turmeric. The anti-oxidant principle was found to be a heat stable protein. Trypsin treatment abolished the anti-oxidant activity. The anti-oxidant principle had an absorbance maximum at 280 nm. After gel filtration, the protein showed a 2-fold increase in anti-oxidant activity and showed 2 bands in the SDS-PAGE with approximate molecular weight range of 24,000 Da. The protein showed a concentration-dependent inhibitory effect on the promoter induced lipid peroxidation. A 50% inhibitory activity of lipid peroxidation was observed at a protein concentration of 50 micrograms/ml. Ca(2+)-ATPase of rat brain homogenate was protected to nearly 50% of the initial activity from the lipid peroxidant induced inactivation by this protein. This protection of Ca(2+)-ATPase activity was found to be associated with the prevention of loss of -SH groups.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / chemistry
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Antioxidants / pharmacology*
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Brain / drug effects
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Brain / enzymology
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Calcium-Transporting ATPases / metabolism
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Chromatography, Gel
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Cod Liver Oil / metabolism
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Curcumin / chemistry
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Curcumin / pharmacology*
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Dose-Response Relationship, Drug
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Electrophoresis, Polyacrylamide Gel
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Hot Temperature
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Lipid Peroxidation / drug effects*
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Male
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Molecular Weight
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Plant Oils / chemistry
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Plant Oils / isolation & purification
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Plant Oils / pharmacology*
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Plant Proteins / chemistry
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Plant Proteins / pharmacology*
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Rats
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Rats, Wistar
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Spectrophotometry, Ultraviolet
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Sulfhydryl Compounds / metabolism
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Thiobarbituric Acid Reactive Substances / metabolism
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Trypsin / metabolism
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Trypsin / pharmacology
Substances
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Antioxidants
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Plant Oils
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Plant Proteins
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Sulfhydryl Compounds
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Thiobarbituric Acid Reactive Substances
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Cod Liver Oil
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Trypsin
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Calcium-Transporting ATPases
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Curcumin