Abstract
The synthetic analogue of phosphatidylserine, cholesterylphosphorylserine (CPHS) inhibits T-cell-mediated immune responses in mice. Tested in cultured mouse spleen cells, CPHS inhibits concanavalin A-induced activation of DNA synthesis (IC50, 3.5 microM). Injected i.p. during the efferent phase, CPHS (25-100 mg/kg) inhibits the manifestations of delayed-type of hypersensitivity. The compound (25 mg/kg i.p., daily) reduces the acute graft-versus-host reaction when given for 5 days to donor mice before the isolation of spleen cells used for the inoculum. These data suggest that the addition of a phosphorylserine group to a steroid ring may produce immunoregulatory compounds.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adjuvants, Immunologic / pharmacology*
-
Animals
-
Cholesterol / analogs & derivatives*
-
Cholesterol / immunology
-
Cholesterol / pharmacology*
-
Graft vs Host Reaction / drug effects
-
Hypersensitivity, Delayed / prevention & control
-
Lymphocyte Activation / drug effects
-
Male
-
Mast Cells / drug effects
-
Mice
-
Mice, Inbred BALB C
-
Mice, Inbred C57BL
-
Phosphatidylserines / immunology
-
Phosphatidylserines / pharmacology*
-
Phosphoserine / analogs & derivatives*
-
Phosphoserine / immunology
-
Phosphoserine / pharmacology
-
Spleen / cytology
-
Spleen / drug effects
-
T-Lymphocytes / drug effects*
Substances
-
Adjuvants, Immunologic
-
Phosphatidylserines
-
cholesterylphosphorylserine
-
Phosphoserine
-
Cholesterol