Abstract
The saponin fraction prepared from the methanolic extract of Allium chinense bulbs exhibited inhibitory activities on cyclic AMP phosphodiesterase (cAMP PDE) (43.5%) and Na+/K+ ATPase (59.3%) at a sample concentration of 100 micrograms ml-1, respectively. Attempted purification of the active fraction through column chromatography on silica gel and ODS silica gel resulted in the isolation of six steroidal saponins, one of which appeared to be a new compound and one to be the first isolation from a natural source. (25R,S)-5 alpha-Spirostan-3 beta-ol tetrasaccharide showed inhibitory activities on both cAMP PDE and Na+/K+ ATPase, while (25R)-3 beta-hydroxy-5 alpha-spirostan-6-one di- and tri-saccharides inhibited only cAMP PDE.
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors*
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Allium / chemistry*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology
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Magnetic Resonance Spectroscopy
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Medicine, Chinese Traditional
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Molecular Sequence Data
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Phytosterols / chemistry
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Phytosterols / isolation & purification*
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Phytosterols / pharmacology
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Phytotherapy
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Saponins / chemistry
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Saponins / isolation & purification*
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Saponins / pharmacology
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Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors*
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Infrared
Substances
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Enzyme Inhibitors
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Phytosterols
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Saponins
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3',5'-Cyclic-AMP Phosphodiesterases
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Sodium-Potassium-Exchanging ATPase