The modulus of elasticity of periodontal membrane, as an essential parameter indicating the mechanical character of periodontal membrane, is very important in biomechanical studies (e.g. finite element stress analysis and photoelastic analysis) of dental restoration. However, determination of modulus of elasticity of human periodontal membrane has not been reported in China, and there are differences among the foreign reports. In this study, 135 periodontal samples were collected from fresh teeth and alveoli of 6 healthy adults. Drawing and compressing test were performed. The results showed that the tensile modulus of elasticity of human periodontal membrane is between 3.35 and 4.59MPa and the compress modulus of elasticity 0.5402(+/- 0.348) MPa.