Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk

Appl Environ Microbiol. 1995 Aug;61(8):3177-9. doi: 10.1128/aem.61.8.3177-3179.1995.

Abstract

Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestion profiles identical to that of the chocolate milk isolate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Animals
  • Cacao
  • Child
  • Child, Preschool
  • DNA Fingerprinting
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Disease Outbreaks
  • Electrophoresis, Gel, Pulsed-Field / methods*
  • Female
  • Humans
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / isolation & purification
  • Listeriosis / epidemiology
  • Listeriosis / microbiology*
  • Male
  • Middle Aged
  • Milk / microbiology*
  • Molecular Epidemiology
  • Wisconsin / epidemiology

Substances

  • DNA, Bacterial