DNA-like duplexes with repetitions. I. Properties of concatemer duplexes formed by d(T-G-C-A-C-A-T-G)

Nucleic Acids Res. 1980 Jun 11;8(11):2413-29. doi: 10.1093/nar/8.11.2413.

Abstract

A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the following: delta Ho1 = -9.2 +/- 0.3 kcal/mol, delta So1 = -27 +/- 1 e.u. The data obtained show that pseudopolymeric duplexes having structures that are similar to DNA-B-type helices are formed in solutions of d(T-G-C-A-C-A-T-G). Polymerization of 32P-d(pT-G-C-A-C-A-T-G) induced by water-soluble carbodiimide has been carried out under the conditions of concatemer stability. The yield of the dimer, a 16-member oligonucleotide, was 13%.

MeSH terms

  • Carbodiimides
  • Chemical Phenomena
  • Chemistry
  • Circular Dichroism
  • DNA / chemical synthesis*
  • Macromolecular Substances
  • Nucleic Acid Conformation
  • Repetitive Sequences, Nucleic Acid*
  • Spectrophotometry, Ultraviolet
  • Thermodynamics

Substances

  • Carbodiimides
  • Macromolecular Substances
  • DNA