Detection of plant virus by using purified IgG in ELISA

J Virol Methods. 1981 Jul;3(1):27-35. doi: 10.1016/0166-0934(81)90019-7.

Abstract

An optimized ELISA system for the detection of soybean mosaic virus was developed. Purified anti-soybean mosaic virus IgG, a secondary ovalbumin coat, and a clearly defined optimal concentration of enzyme-conjugated anti-soybean mosaic virus IgG are used. The test detects purified virus, or purified virus added to leaf and seed extracts, at a concentration of 2.5 ng/ml. Various buffers significantly affect detection sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral
  • Enzyme-Linked Immunosorbent Assay
  • Glycine max
  • Immunoglobulin G
  • Microbiological Techniques*
  • Mosaic Viruses / immunology
  • Mosaic Viruses / isolation & purification*

Substances

  • Antibodies, Viral
  • Immunoglobulin G