Quantitative comparisons of the lactate and acetate produced by human dental plaque in vitro and in situ were made before and after five-minute exposures to 1% NaF gel. Assays included total ionic plaque fluoride by a fluoride electrode, L(+)- and D(-)-lactate by an enzyme method, and acetate by a standard GLC procedure. A single topical application of NaF gel increased plaque fluoride about eight-fold. Six hours after gel use, plaque fluoride had declined to about 20% above pretreatment levels. Plaque fluoride baseline levels and variation between and within subjects were greater than expected. This may have been due largely to non-standardized oral hygiene practice and/or the routine use of fluoride dentifrices and the wide variation in the natural fluoride content of drinking water. Fluoride gel use significantly reduced L(+)-lactate in vitro, but D(-)-lactate and acetate were virtually unaffected. Conversely, gel use significantly inhibited the in situ production of each of these acids. The findings of this study indicate that topically applied fluoride gel impairs plaque acidogenesis to an extent that could be meaningful in preventing dental caries.