Abstract
Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solubilized preparation of the carboxylase and is an apparent competitive inhibitor of the carboxylation of Phe-Leu-Glu-Glu-Leu.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Carboxyglutamic Acid / biosynthesis
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Amino Acid Sequence
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Animals
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Carbon-Carbon Ligases*
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Kinetics
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Ligases / antagonists & inhibitors*
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Microsomes, Liver / enzymology*
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Oligopeptides / chemical synthesis
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Peptide Fragments / pharmacology
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Rats
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Structure-Activity Relationship
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Vitamin K / metabolism*
Substances
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Oligopeptides
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Peptide Fragments
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Vitamin K
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1-Carboxyglutamic Acid
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Ligases
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Carbon-Carbon Ligases
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glutamyl carboxylase