Piglets were weaned from multiparous sows at 10 days (Group E; N = 7) or 35 days (Group C; N = 8) of lactation. Blood samples were collected at 8 h intervals from the day before weaning (Day--1) until and including the day of first mating, then once daily until 10 days post coitum. Additionally, 5 sows in each group were sampled at 30 min intervals for 12 h on Days--1, 0 and 1, then at 30 min intervals for 6 h daily until mating, finally at 30 min intervals for 2 h daily until 5 days post coitum. Group E sows had relatively longer weaning to remating intervals (8.3 +/- 0.8 compared with 5.0 +/- 0.7 days; P less than 0.01) and tended, but not significantly, to produce smaller subsequent litters (10.2 +/- 1.9 compared with 12.0 +/- 0.6). Sows in group E had lower lactational and post-weaning plasma LH levels (P less than 0.001). They also had greatly attenuated preovulatory LH rises and the area under the peak was reduced (P less than 0.01 and P less than 0.001, respectively). Preweaning plasma prolactin levels were higher at 9 days of lactation than at 34 days and levels in both groups dropped precipitously subsequent to piglet removal. Although peak levels of prolactin at oestrus did not differ between treatments, they tended to occur before the LH peak in sows of Group C and after the LH peak in Group E.(ABSTRACT TRUNCATED AT 250 WORDS)