A simple procedure for preparing and staining undecalcified sections of avian growth plate and metaphysis

Avian Dis. 1984 Jan-Mar;28(1):285-8.

Abstract

This report describes an inexpensive, rapid technique for preparing 4-micron-thick undecalcified sections of avian growth plate and metaphysis using paraffin embedment, rotary microtomes, and disposable knives. Sections were stained by von Kossa's technique with a hematoxylin-and-eosin counterstain.

MeSH terms

  • Animals
  • Chickens / anatomy & histology*
  • Growth Plate / cytology
  • Growth Plate / metabolism
  • Histocytochemistry
  • Histological Techniques / veterinary*
  • Minerals / metabolism
  • Tibia / cytology

Substances

  • Minerals