The distribution of fibronectin (FN) and laminin (LM) in basal cell epithelioma was evaluated by indirect immunofluorescence. FN is a glycoprotein which promotes interaction between cells and the extracellular matrix, and is present at the dermal-epidermal junction (DEJ) and throughout the dermis, but absent in the normal epidermis. LM, a noncollagenous basement membrane protein, plays a role in epithelial adhesion to type IV collagen, and is normally present in the DEJ, but absent in the epidermis. The role of FN and LM in epithelial differentiation has not been established. Therefore, the distribution of FN and LM in a tumor of epithelial origin was studied by indirect immunofluorescence. Using affinity-purified antibodies to FN and LM, and the appropriate fluorescein-conjugated second antibodies, normal skin and 7 basal cell tumors were examined. By immunofluorescence, nests of malignant basal cells were surrounded by linear LM staining. FN immunofluorescence was intense throughout the connective tissue stroma of all tumors. Five tumors also showed FN staining within the nests of neoplastic cells, and 4 of these were also LM-positive in the tumor bulk. These immunofluorescent findings suggest that epidermal neoplasia can be associated with alterations in the distribution of FN and LM.