Effect of dimethyl sulfate on the secondary structure of DNA

Experientia. 1978 Mar 15;34(3):392-4. doi: 10.1007/BF01923056.

Abstract

Hydroxyapatite chromatography has been used to demonstrate that alkylation of DNA at neutral pH may lead to denaturation under conditions where no significant depurination occurs. Presence of salt has a preventive effect on such denaturation.

MeSH terms

  • Alkylating Agents
  • Chromatography
  • DNA*
  • Hydroxyapatites
  • Methane / analogs & derivatives*
  • Nucleic Acid Denaturation*
  • Sulfuric Acid Esters
  • Sulfuric Acids*

Substances

  • Alkylating Agents
  • Hydroxyapatites
  • Sulfuric Acid Esters
  • Sulfuric Acids
  • DNA
  • dimethyl sulfate
  • Methane