Abstract
Hydroxyapatite chromatography has been used to demonstrate that alkylation of DNA at neutral pH may lead to denaturation under conditions where no significant depurination occurs. Presence of salt has a preventive effect on such denaturation.
MeSH terms
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Alkylating Agents
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Chromatography
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DNA*
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Hydroxyapatites
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Methane / analogs & derivatives*
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Nucleic Acid Denaturation*
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Sulfuric Acid Esters
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Sulfuric Acids*
Substances
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Alkylating Agents
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Hydroxyapatites
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Sulfuric Acid Esters
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Sulfuric Acids
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DNA
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dimethyl sulfate
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Methane