Five species (28 strains) of actinomycetes isolated from tobacco were tested for antagonism against 12 species of storage and field fungi associated with tobacco. Two strains of Streptomyces albus were antagonistic against all test fungi. The actinomycetes grew more rapidly, produced more pigment, and had more pronounced antibiotic activity when grown at 36 C than at 28 C. Krasilnikov's synthetic medium, SMK-1, supported the greatest antifungal activity. More of the actinomycetes were antagonistic against more test fungi when grown for 20 days rather than 10 days.