A simple screening technique for the detection of Pseudomonas aeruginosa colonies by their fluorescence on MacConkey agar under ultraviolet light is proposed. From 306 nonlactose fermenting cultures screened under the ultraviolet light, 108 fluorescent isolates were obtained. These were screened biochemically, with 103 (94.8%) being verified as P. aeruginosa. From the 198 nonfluorescing cultures, only one suspected P. aeruginosa was isolated.