Expression of octopamine/tyramine receptors and immune regulation in Litopenaeus vannamei under acute and chronic thermal stress

Li-Yang Hsu; Hsin-Wei Kuo; Winton Cheng

doi:10.1016/j.dci.2024.105195

Expression of octopamine/tyramine receptors and immune regulation in Litopenaeus vannamei under acute and chronic thermal stress

Dev Comp Immunol. 2024 May 17:158:105195. doi: 10.1016/j.dci.2024.105195. Online ahead of print.

Authors

Li-Yang Hsu¹, Hsin-Wei Kuo², Winton Cheng³

Affiliations

¹ Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC.
² General Research Service Center, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC.
³ Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC. Electronic address: winton@mail.npust.edu.tw.

PMID: 38762098
DOI: 10.1016/j.dci.2024.105195

Abstract

This study investigated the impact of hyperthermal (34 °C) and hypothermal (14 °C) stress on the expression of the octopamine/tyramine receptor (LvOA/TA-R) and immune parameters in Litopenaeus vannamei, which is a species critical to the aquaculture industry. Given the sensitivity of aquatic organisms to climate change, understanding the physiological and immune responses of L. vannamei to temperature variations is essential for developing strategies to mitigate adverse effects. This research focuses on the immune response and expression changes of LvOA/TA-R under acute (0.5, 1, and 2 h) and chronic (24, 72, and 168 h) thermal stress conditions. Our findings reveal that thermal stress induces changes in LvOA/TA-R expression and impacts immune responses. Immune parameters such as total haemocyte count, differential haemocyte count, phenoloxidase activity, respiratory bursts, lysozyme activity, clearance efficiency, and phagocytosis exhibited a general trend of significant decline under the stress conditions. LvOA/TA-R had a higher expression in haemocyte under hyperthermal stress. The study elucidated that thermal stress modifies the expression of the LvOA/TA-R and diminishes immune functionality in L. vannamei, underscoring the potential influence of climate change on industry.

Keywords: Climate change; Immune response; Litopenaeus vannamei; Octopamine/tyramine receptor; Sustainable aquaculture; Thermal stress.