Regulatory role of BNP in the spinal center of rats with nonbacterial prostatitis

Xiaorong Yang; Jieping Hu; Qiang Chen; Lin Yang; Yifu Liu; Longhua Luo; Ming Ma; Wenjie Xie; Binbin Gong; Ting Sun; Jiazhen Tang; Fang Liu

doi:10.3233/THC-248031

Regulatory role of BNP in the spinal center of rats with nonbacterial prostatitis

Technol Health Care. 2024 Apr 15. doi: 10.3233/THC-248031. Online ahead of print.

Authors

Xiaorong Yang^{1

1}, Jieping Hu^{1

1}, Qiang Chen^{1

1}, Lin Yang¹, Yifu Liu¹, Longhua Luo¹, Ming Ma¹, Wenjie Xie¹, Binbin Gong¹, Ting Sun¹, Jiazhen Tang², Fang Liu²

Affiliations

¹ Department of Urology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.
² Department of Health Management Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.

PMID: 38759060
DOI: 10.3233/THC-248031

Abstract

Background: A growing body of evidence has shown that activating spinal cord glial cells (typically astrocytes and microglial cells) is closely related to hyperpathia and persistent pain.

Objective: To investigate the expression of GFAP and CR3/CD11b in cornu dorsale medullae spinalis of rats with nonbacterial prostatitis, to explore the therapeutic efficacy and action mechanism of intrathecal injection of BNP alleviating chronic neuropathic pain.

Methods: Eighteen male SPF SD rats were randomly divided into sham operation control group, nonbacterial prostatitis group (NBP) and intrathecal injection BNP group, the NBP model was established by intraprostatic injection of CFA, and the spinal cord of L6-S1 segment was extracted seven days after intrathecal injection of BNP; The expression of GFAP and CR3/CD11b in dorsal horn of spinal cord were detected by immunofluorescence and Western blot.

Results: The cumulative optical density values of GFAP and CR3/CD11b immunofluorescence assay in the NBP group were higher than those in the sham operation group, with statistical significance (p⁢ï⁢¼⁢ 0.01); The expression of GFAP and CR3/CD11b in intrathecal injection BNP group were lower than those in NBP group, the differences were statistically significant (p⁢ï⁢¼⁢ 0.01). Western blot results showed that the expression of GFAP and CR3/CD11B in NBP group were higher than those in sham operation group, with statistical significance (p⁢ï⁢¼⁢ 0.05). The expression of GFAP and CR3/CD11B in intrathecal injection BNP group were lower than those in NBP group, the differences were statistically significant (p⁢ï⁢¼⁢ 0.05).

Conclusion: Intrathecal injection of BNP can down-regulate the expressions of GFAP and CR3/CD11b in L6-S1 spinal cord of NBP rat model and to further inhibit chronic pain caused by NBP.

Keywords: CR3/CD11b; GFAP; Nonbacterial prostatitis BNP.