Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Miaomiao Xiong; Xiaomin Yang; Lan Yao; Zhuang Li; Jinxiu Zhang; Jianhua Lv

doi:10.3389/fmicb.2024.1408013

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Front Microbiol. 2024 May 2:15:1408013. doi: 10.3389/fmicb.2024.1408013. eCollection 2024.

Authors

Miaomiao Xiong¹, Xiaomin Yang¹, Lan Yao², Zhuang Li¹, Jinxiu Zhang¹, Jianhua Lv¹

Affiliations

¹ College of Life Sciences, Hebei Normal University, Shijiazhuang, China.
² Institute of Biology, Hebei Academy of Science, Shijiazhuang, China.

Abstract

In order to control the occurrence of ginseng root rot caused by Fusarium solani (Mart.) Sacc., the antifungal compounds of the mushroom Suillus bovinus were investigated. And three new alkaloids (1-3), named bovinalkaloid A-C, along with one known analog (4), were isolated and identified by bioassay-guided isolation and spectroscopic analyses. Compound 1 strongly inhibited the mycelial growth and spore germination of F. solani with minimum inhibitory concentration of 2.08 mM. Increases in electrical conductivity, nucleic acid, and protein contents, and decreases in lipid content showed that the membrane permeability and integrity were damaged by compound 1. Compound 1 also increased the contents of malondialdehyde and hydrogen peroxide and the activities of antioxidant enzymes, indicating that lipid peroxidation had taken place in F. solani. Compound 1 may serve as a natural alternative to synthetic fungicides for the control of ginseng root rot.

Keywords: Fusarium solani; Suillus bovinus; alkaloids; antifungal activities; mushrooms.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. Science Research Start-up Fund for Doctor of Hebei Normal University, grant numbers L2023B22 and L2024B16.