Introduction: Oral diseases affect billions worldwide, with dental caries being a significant concern. Silver-copper nanoparticles (Ag-Cu NPs) synthesized from pitaya plant extract offer a potential solution due to their antimicrobial properties. This study aimed to evaluate the inhibitory effects of Ag-Cu NPs on Streptococcus mutans (S. mutans), a major contributor to dental caries.
Methodology: Ag-Cu NPs were synthesized using a green chemical method and characterized using a scanning electron microscope (SEM), ultraviolet-visible (U-vis) spectrophotometer, and Fourier Transform Infrared Spectroscopy (FT-IR). The minimum inhibitory concentration (MIC) against S. mutans was determined using the broth microdilution method, while the antibiofilm effect was assessed by the crystal violet method.
Results: The synthesized Ag-Cu NPs demonstrated antimicrobial activity with an MIC of 128 µg/ml, significantly inhibiting S. mutans biofilm formation by up to 94% at a concentration of 256 µg/ml. Characterization studies confirmed the successful synthesis of Ag-Cu NPs with spherical morphology.
Conclusions: These findings highlight the potential of Ag-Cu NPs as a novel approach for combating dental caries by targeting S. mutans biofilms. Further research is warranted to explore their safety and efficacy in clinical settings.
Keywords: antibiofilm assay; antimicrobial effect; copper nanoparticles; silver nanoparticles; dental caries.
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