Temperature rise and its influence on the toxic effects caused by cyanotoxins in a neotropical catfish

Maiara Vicentini; Sabrina L M Calado; Jacqueline B K Pessati; Maiara C Perussolo; Juliana R Lirola; Fellip R Marcondes; Natália do Nascimento; Camila L Beghetto; Mauro C P Vilar; Maritana Mela; Lucila A A Coral; Valéria F Magalhães; Viviane Prodocimo; Marta M Cestari; Helena C Silva de Assis

doi:10.1016/j.envpol.2024.124166

Temperature rise and its influence on the toxic effects caused by cyanotoxins in a neotropical catfish

Environ Pollut. 2024 May 14:353:124166. doi: 10.1016/j.envpol.2024.124166. Online ahead of print.

Authors

Maiara Vicentini¹, Sabrina L M Calado¹, Jacqueline B K Pessati², Maiara C Perussolo³, Juliana R Lirola⁴, Fellip R Marcondes⁴, Natália do Nascimento⁵, Camila L Beghetto⁶, Mauro C P Vilar⁷, Maritana Mela⁸, Lucila A A Coral⁶, Valéria F Magalhães⁷, Viviane Prodocimo⁵, Marta M Cestari⁴, Helena C Silva de Assis⁹

Affiliations

¹ Post Graduation Program in Ecology and Conservation, Federal University of Paraná, Brazil.
² Pharmacology Department, Federal University of Paraná, Brazil.
³ Pharmacology Department, Federal University of Paraná, Brazil; Pelé Pequeno Príncipe Research Institute, Brazil.
⁴ Genetics Department, Federal University of Paraná, Brazil.
⁵ Physiology Department, Federal University of Paraná, Brazil.
⁶ Chemistry and Biology Department, Federal University of Technology - Paraná, Brazil.
⁷ Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Brazil.
⁸ Cell Biology Department, Federal University of Paraná, Brazil.
⁹ Pharmacology Department, Federal University of Paraná, Brazil. Electronic address: helassis@ufpr.br.

PMID: 38754694
DOI: 10.1016/j.envpol.2024.124166

Abstract

Potentially toxic cyanobacterial blooms (cyanoHABs) have become a problem in public water supply reservoirs. Temperature rise caused by climate change can increase the frequency and intensity of blooms, which may influence the cyanotoxins concentration in the environment. This study aimed to evaluate the effect of the temperature on the responses of a Neotropical catfish exposed to a neurotoxin-rich cyanobacterial crude extract (Raphidiopsis raciborskii T3). Juveniles of Rhamdia quelen were exposed to four treatments, based on study data: control at 25 °C (C25), control at 30 °C (C30), crude extract equivalent to 10⁵ cells.mL^-l of R. raciborskii at 25 °C (CE25) and 30 °C (CE30). After 96 h of exposure, the fish were anesthetized and blood was taken. After euthanasia, the gill, posterior kidney, brain, muscle, liver and gonad were sampled for hematological, biochemical, genotoxic and histopathological biomarker analysis. Liver was sampled for proteomic analysis for identification of proteins related to energy production. Water samples were collected at the beginning and the end of the experiment for neurotoxins quantification. Different parameters in both males and females were altered at CE25, evidencing the effects of neurotoxins in freshwater fish. At CE30, a water warming scenario, more effects were observed in females than at 25 °C, such as activation of saxitoxin metabolism pathway and genotoxicity. More damage to macromolecules was observed in females at the higher temperature, demonstrating that the increase in temperature can aggravate the toxicity of neurotoxins produced by R. raciborskii T3.

Keywords: CyanoHABs; Global warming; Rhamdia quelen; Saxitoxins; Toxicity.