Comparative analysis of stripe rust resistance in seedling stage and Yr gene incidence in spring and winter wheat from Xinjiang, China

Hanlin Lai; Yuyang Shen; Hong Yang; Dilantha W G Fernando; Chenrong Ren; Feifei Deng; Yi Lu; Na Sun; Li Chen; Guangkuo Li; Huiqing Wang; Haifeng Gao; Yue Li

doi:10.3389/fpls.2024.1394213

Comparative analysis of stripe rust resistance in seedling stage and Yr gene incidence in spring and winter wheat from Xinjiang, China

Front Plant Sci. 2024 May 1:15:1394213. doi: 10.3389/fpls.2024.1394213. eCollection 2024.

Authors

Hanlin Lai^{1

2}, Yuyang Shen², Hong Yang², Dilantha W G Fernando³, Chenrong Ren⁴, Feifei Deng², Yi Lu⁴, Na Sun⁵, Li Chen², Guangkuo Li², Huiqing Wang⁴, Haifeng Gao², Yue Li¹

Affiliations

¹ College of Life Science, Xinjiang Agricultural University, Urumqi, China.
² Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Urumqi, Xinjiang, China.
³ Department of Plant Sciences, University of Manitoba, Winnipeg, MB, Canada.
⁴ Plant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.
⁵ Institute of Agricultural Sciences of Ili Prefecture, Ili, Xinjiang, China.

Abstract

Background: Stripe rust, caused by the fungus Puccinia striiformis f.sp. tritici (Pst), poses a significant threat to global wheat production.

Objectives: This study aims to analyze the distribution of stripe rust resistance genes, characterize resistance phenotypes at the seedling stage of 137 spring and 149 winter wheat varieties in Xinjiang, China, and discern differences in resistance between spring and winter wheat varieties.

Design: We used various Pst races (CYR23, CYR29, CYR31, CYR32, CYR33, CYR34) to characterize seedling resistance of spring and winter wheat varieties and to correlate resistance to the presence of wheat resistance genes (Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr41, Yr80, Yr81) using molecular markers.

Results: Among spring wheat varieties, 62, 60, 42, 26, 51, and 24 varieties exhibited resistance to CYR23, CYR29, CYR31, CYR32, CYR33, and CYR34, respectively, with four varieties resistant to all varieties. Among winter wheat varieties, 66, 32, 69, 26, 83, 40 varieties demonstrated resistance to CYR23, CYR29, CYR31, CYR32, CYR33, and CYR34, respectively, with four varieties resistant to all varieties. Molecular testing revealed that, in spring wheat, 2, 17, 21, 61, 10, 0, 10, 79, and 32 varieties carried Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr41, Yr80, and Yr81 genes, respectively. In winter wheat, 40, 20, 7, 143, 15, 1, 6, 38, and 54 varieties carried Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr41, Yr80, and Yr81 genes, respectively. Notably, winter wheat exhibited a significantly higher resistance frequency than spring wheat, particularly in the incidence of Yr9, Yr10, Yr17, Yr18, and multi-gene combinations.

Conclusion: In summary, this study provides information on seedling stage resistance to stripe rust 286 Xinjiang wheat varieties, elucidates the distribution of resistance genes in this population, and offers a mechanistic basis for breeding durable resistance in wheat. varieties from Xinjiang.

Keywords: Puccinia striiformis f. sp. tritici; Xinjiang; disease resistance; molecular detection; wheat variety.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This project was supported by the Science and Technology Assistance Project of Xinjiang Uygur Autonomous Region (2022E02070); Regional Coordinated Innovation Project, Shanghai Cooperation Organization Science and Technology Partnership Program (2023E01015); Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01A135); Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis (KFJJ202104); Third Tianshan Talent Plan of Xinjiang Uygur Autonomous Region (2022TSYCCX0081); Earmarked Fund for XJARS-01; Science and Technology Major Project of Xinjiang Uygur Autonomous Region (2023A02009); Urumqi Integrated Experimental Station of China Agriculture Research System for Wheat (CARS-03-88); and Project of Fund for Stable Support to Agricultural Sci-Tech Renovation (XJNKYWDZC-2023004-1).