Carbon dots (CDs) are a novel category of carbon-based nanomaterials characterized by their small size, often less than 10 nm. CDs physical, chemical, and optical properties can be tuned using one-pot assembly. Because of their non-toxicity, biocompatibility, chemical and physical responsiveness, photo- and chemical-bleaching resistance, and low cost, nanoparticles have become incredibly versatile. They find various applications in detecting inorganic substances, bio sensing, visualizing cells, studying biological processes in live cells, and aiding in medication delivery. Additionally, CDs exhibit versatility in electronics and energy storage, making them promising candidates for applications in solar cells, light-emitting diodes, and supercapacitors. CDs are more photo stable for hours than typical fluorescent semiconductor quantum dots. Before applying CDs, they must be characterized. Techniques such as UV-VIS spectroscopy, fluorescence spectrophotometry, FT-IR, TEM, XRD, Raman spectroscopy, and NMR are commonly used to assess their photophysical and structural properties. This article review explores the synthesis, characterization applications of CDs in analytical techniques for the determination of various analytes. The article provides a detailed analysis of the different methodologies used to make nanomaterials and devices for the characterization of CDs. It also discusses the challenges that arise when using CDs in analytical techniques for detecting different analytes. The focus of this review is on accurately determining pharmaceutical and biological samples using CDs as sensing probes.
Keywords: Analytical techniques; Biomedical applications; Carbon dots; Nanomaterial-based sensing; Sensing probes.
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